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Figure 2 | Veterinary Research

Figure 2

From: Identification of two residues within the NS1 of H7N9 influenza A virus that critically affect the protein stability and function

Figure 2

H7N9 NS1-S212P mutation reduces its capacity to inhibit WSN and PR8 delNS1 virus-induced host antiviral response. A 293T cells were transfected with plasmids expressing H7N9 NS1-WT (WT), NS1 mutants (R55E, H63Q, E70K, P87S, S114P, A143T, I178V, S212P) or EV for 24 h, then the cells were infected with WSN virus (MOI = 1) for 12 h, followed by quantitative real-time PCR to detect IFN-β mRNA expression levels. BG After 293T cells were transfected with plasmids expressing H7N9 NS1-WT (WT), NS1-S212P (S212P) or EV for 24 h, the cells were then infected with WSN or PR8 delNS1 virus (MOI = 1) for 12 h, followed by quantitative real-time PCR to detect IFN-α (B), IFN-β (C), IL-28 (D), OASL (E), MxA (F) and IL-6 (G) mRNA levels. In each experiment, the value observed in mock-infected cells was normalized to 1. Plotted are the average levels from three independent experiments. The error bars represent the s.e.m. *P < 0.05, **P < 0.01.

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