Salmonella Gallinarum delivering M2eCD40L in protein and DNA formats acts as a bivalent vaccine against fowl typhoid and H9N2 infection in chickens

Table 2 Mortality and bacterial load in the chickens post-challenge

Groups^a	Challenge^b			Liver^e	Spleen^e
Groups^a	Mortality^c	Liver^d	Spleen^d	Liver^e	Spleen^e
PBS	6/8*	2/2*	2/2*	3.44 ± 2.42*	2.8 ± 1.84*
JOL916	1/8	4/7	0/7	2.88 ± 2.13	0
JOL2074	1/8	5/7	1/7	2.92 ± 2.20	0.75 ± 1.17
JOL2074 + JOL2076	1/8	4/7	0/7	2.75 ± 2.09	0

^aFour weeks old chickens (n = 8) were orally vaccinated with PBS, JOL916, JOL2074 or JOL2074 + JOL2076.
^bChallenge was performed with a wild-type SG strain using 1.6 × 10⁶ CFU/bird after 28 days post-vaccination.
^cNumber of dead birds upon challenge.
^dNumber of birds positive for SG.
^eBacterial load in liver (CFU/gram) and spleen (CFU/spleen). *p < 0.05.

ISSN: 1297-9716