Table 2 Gradient program employed for the separation of PTC-amino acids
Time (min) | 0 | 10 | 20 | 21 | 34 | 35 | 43 | 44 | 49 | 50 | 53 |
|---|---|---|---|---|---|---|---|---|---|---|---|
A% (v/v) | 95 | 91 | 89 | 79 | 77 | 75 | 68 | 20 | 10 | 95 | 95 |
B% (v/v) | 5 | 9 | 11 | 21 | 23 | 25 | 32 | 80 | 90 | 5 | 5 |
Time (min) | 0 | 10 | 20 | 21 | 34 | 35 | 43 | 44 | 49 | 50 | 53 |
|---|---|---|---|---|---|---|---|---|---|---|---|
A% (v/v) | 95 | 91 | 89 | 79 | 77 | 75 | 68 | 20 | 10 | 95 | 95 |
B% (v/v) | 5 | 9 | 11 | 21 | 23 | 25 | 32 | 80 | 90 | 5 | 5 |
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