Figure 5

Bacterial adherence and invasion assays. Strains CH3 and RA1062 were tested on Vero cells. A Adherence assay; B Invasion assay. The data represent the number of bacteria bound to or invaded into Vero cells in each well of a 24-well plate. The error bars represent mean ± standard deviations from three independent experiments (**p < 0.01; ***p < 0.001). The adherence and invasion capacities of the RA1062 mutant were significantly increased in comparison with its WT strain CH3.