Strains/plasmids | Description | References |
---|---|---|
Strains | ||
 Χ232 | E. coli Δasd strain used for cloning of genes into asd+ plasmid | [28] |
 BL21(DE3)pLysS | F−, ompT, hsdSB (r −B , m −B ), dcm, gal, λ (DE3), pLysS, Cmr | Promega, USA |
 JOL967 | ∆lon, ∆cpxR and ∆asd mutant of SG | [28] |
 JOL916 | ∆lon and ∆cpxR mutant of SG | [28] |
 JOL2068 | JOL967 with pJHL65 plasmid | This study |
 JOL2077 | JOL967 with pJHL65-S1 plasmid | This study |
 JOL1992 | BL21(DE3)pLysS with pET28a-S1 plasmid | This study |
Plasmids | ||
 pJHL65 | An asd+ vector, pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid, 6xHis, high copy number |  |
 pJHL65-S1 | pJHL65 carrying IBV S1 gene sequence | This study |
 pET28a (+) | IPTG-inducible expression vector; Kanr | Novagen, USA |
 pET28a-S1 | pET28a plasmid carrying S1 gene | This study |