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Table 1 Bacterial strains and plasmids used in this study

From: Oral immunization with a novel attenuated Salmonella Gallinarum encoding infectious bronchitis virus spike protein induces protective immune responses against fowl typhoid and infectious bronchitis in chickens

Strains/plasmids Description References
 Χ232 E. coli Δasd strain used for cloning of genes into asd+ plasmid [28]
 BL21(DE3)pLysS F, ompT, hsdSB (r B , m B ), dcm, gal, λ (DE3), pLysS, Cmr Promega, USA
 JOL967 ∆lon, ∆cpxR and ∆asd mutant of SG [28]
 JOL916 ∆lon and ∆cpxR mutant of SG [28]
 JOL2068 JOL967 with pJHL65 plasmid This study
 JOL2077 JOL967 with pJHL65-S1 plasmid This study
 JOL1992 BL21(DE3)pLysS with pET28a-S1 plasmid This study
 pJHL65 An asd+ vector, pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid, 6xHis, high copy number  
 pJHL65-S1 pJHL65 carrying IBV S1 gene sequence This study
 pET28a (+) IPTG-inducible expression vector; Kanr Novagen, USA
 pET28a-S1 pET28a plasmid carrying S1 gene This study