Strains
|
Χ232
|
E. coli Δasd strain used for cloning of genes into asd+ plasmid
|
[28]
|
BL21(DE3)pLysS
|
F−, ompT, hsdSB (r
−B
, m
−B
), dcm, gal, λ (DE3), pLysS, Cmr
|
Promega, USA
|
JOL967
|
∆lon, ∆cpxR and ∆asd mutant of SG
|
[28]
|
JOL916
|
∆lon and ∆cpxR mutant of SG
|
[28]
|
JOL2068
|
JOL967 with pJHL65 plasmid
|
This study
|
JOL2077
|
JOL967 with pJHL65-S1 plasmid
|
This study
|
JOL1992
|
BL21(DE3)pLysS with pET28a-S1 plasmid
|
This study
|
Plasmids
|
pJHL65
|
An asd+ vector, pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid, 6xHis, high copy number
| |
pJHL65-S1
|
pJHL65 carrying IBV S1 gene sequence
|
This study
|
pET28a (+)
|
IPTG-inducible expression vector; Kanr
|
Novagen, USA
|
pET28a-S1
|
pET28a plasmid carrying S1 gene
|
This study
|