Oral immunization with a novel attenuated Salmonella Gallinarum encoding infectious bronchitis virus spike protein induces protective immune responses against fowl typhoid and infectious bronchitis in chickens

Table 1 Bacterial strains and plasmids used in this study

Strains/plasmids	Description	References
Strains
Χ232	E. coli Δasd strain used for cloning of genes into asd⁺ plasmid	[28]
BL21(DE3)pLysS	F⁻, ompT, hsdS_B (r ⁻_B , m ⁻_B ), dcm, gal, λ (DE3), pLysS, Cm^r	Promega, USA
JOL967	∆lon, ∆cpxR and ∆asd mutant of SG	[28]
JOL916	∆lon and ∆cpxR mutant of SG	[28]
JOL2068	JOL967 with pJHL65 plasmid	This study
JOL2077	JOL967 with pJHL65-S1 plasmid	This study
JOL1992	BL21(DE3)pLysS with pET28a-S1 plasmid	This study
Plasmids
pJHL65	An asd⁺ vector, pBR ori, β-lactamase signal sequence-based periplasmic secretion plasmid, 6xHis, high copy number
pJHL65-S1	pJHL65 carrying IBV S1 gene sequence	This study
pET28a (+)	IPTG-inducible expression vector; Kan^r	Novagen, USA
pET28a-S1	pET28a plasmid carrying S1 gene	This study

ISSN: 1297-9716