Analysis of cytokine gene expressions in PBMCs after stimulation with S1 antigen. Chickens (N = 15) were vaccinated with sterile PBS, JOL2068 or JOL2077, and PBMCs from vaccinated chickens after 28 days post-vaccination were stimulated with purified S1 antigen for 24 h and then analysed for induction of IFN-γ, IL-4 and IL-10 mRNA transcription levels by qRT-PCR assay. Results are expressed as log10 fold induction in mRNA transcription levels of stimulated PBMCs from vaccinated chickens compared to the unstimulated naïve PBMCs. Gene expressions were normalized to GAPDH and mRNA levels of naive cells were used as the calibrator. Data presented are mean ± SD of six chickens per group. *p < 0.05.