PSaV entry depends on Rab5 and Rab7. A, B LLC-PK cells were transfected with scrambled siRNA or siRNAs against Rab5 (A) or Rab7 (B). Next, cells were exposed to AF594-labeled PSaV particles (approximately 415 particles per cell) for EEA1 colocalization or for LAMP2 colocalization. This experiment was done in triplicate and representative images are shown. The scale bars correspond to 10 μm. The bottom portion displays immunoblots to confirm silencing levels of Rab5 and Rab7 by transfection of each corresponding siRNA. The intensity of each target protein relative to GAPDH was determined by densitometric analysis and is indicated above each lane.