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Figure 2 | Veterinary Research

Figure 2

From: Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II

Figure 2

Small interfering RNAs (siRNAs) against clathrin- and dynamin-mediated endocytosis reduce PSaV infection. A LLC-PK cells transfected with scrambled siRNA (Scram) or siRNAs against clathrin heavy chain (CHC), dynamin II, or caveolin-1 were harvested at 24 and 48 h post-transfection. The down-regulation of each protein by siRNA knock-down was evaluated by western blotting analysis using antibodies specific for each protein. The intensity of each target protein relative to GAPDH was determined by densitometric analysis and is indicated above each lane. The cells transfected with each siRNA and then incubated were infected with PSaV Cowden (B), CVB3 Nancy (C), or rotavirus Wa (D) strains. Infected cells were counted after staining with antibodies specific for each virus, and nuclei were counted after staining with DAPI. For each virus, results are shown as the percentage of infected cells, and normalized to the results obtained in the scrambled siRNA-transfected cells. Data for panels BD are presented as mean ± standard deviation of the mean from three independent experiments. Differences were evaluated using one-way ANOVA. *P < 0.05; **P < 0.001; ***P < 0.0001.

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