Figure 2

The in vivo transcription and expression of TsSP1.2 in mice vaccinated with TsSP1.2 DNA/S. typhimurium. A RT-PCR analysis of TsSP1.2 mRNA transcription. TsSP1.2 mRNA was detected in spleen (lane 1) and MLN (lane 2) of TsSP1.2-immunized mice, either in spleen (lane 3) or in MLN (lane 4) of mice vaccinated with only empty pcDNA3.1, not in spleen (lane 5) and MLN (lane 6) of mice inoculated with only PBS. B IFT detection of TsSP1.2 expression. The expression of TsSP1.2 was detected in spleen (a) and MLN (d) of TsSP1.2-immunized mice by IFT with anti-rTsSP1.2 serum, but not in spleen (c) and MLN (f) by IFT with pre-immune serum. By using anti-rTsSP1.2 serum, no distinct staining was observed in spleen (b) and MLN (e) of mice inoculated with only empty pcDNA3.1. Scale bar: 100 μm.