Replication kinetics of novel reassortant IAVs-S in the guinea pig model. Groups of 6 guinea pigs were intranasally inoculated with 105 TCID50 of either A/swine/Chile/VN1401-4/2014(H1N2), A/swine/Chile/VN1401-274/2014(H1N2), A/swine/Chile/VN1401-559/2014(H1N1) or A/swine/Chile/VN1401-1824/2015(H3N2) viruses. Nasal wash samples were collected on 1, 3, 5, 7, and 9 dpi, and viral titers were determined by TCID50 assay. Data are shown as mean ± SD. Horizontal dashed line represents the limit of detection of the assay (101.5 TCID50/mL).