Figure 6
Comparison of PRRSV infection kinetics in PK15S1–CD163 and PK15S10–CD163 for ten different strains. Immunofluorescence staining for PRRSV was performed in the two cell lines. After 0, 12, 24, 48 and 72 hpi, cells were fixed and stained for infection. The number of infected cells was counted and expressed as percentage of infected cells. The blue line represents infection rate/virus titers in PK15S1–CD163 cells, while the red line represents infection rate/virus titers in PK15S10–CD163 cells. G1s1 = genotype 1 subtype 1; G1s3 = genotype 1 subtype 3; G2 = genotype 2; S1 = PK15S1–CD163; S10 = PK15S10–CD163. Scale bar: 100 µm.