Figure 5

DNA fragmentation of M. bovis-infected macrophages was not inhibited by 3-ABA treatment. Bovine macrophages were pretreated with 50 µM of 3-ABA before M. bovis infection with AN5 strain (10:1) during 16 h. Quantification of DNA fragmentation shows mean fluorescence intensity (MFI) fold change of M. bovis-infected and staurosporine-treated (0.5 µM) macrophages with respect to uninfected cells. The results are representative of three independent experiments. Asterisk represent a p value equal or less than 0.05 of statistical significance.