Figure 3
Cyclosporine A (CsA) inhibits AIF and Endo G nuclear translocation and DNA fragmentation of M. bovis-infected macrophages. Bovine macrophages were pretreated with 1 µM of CsA before M. bovis infection with AN5 strain (10:1) during 16 h. Concentration of A AIF, B Endo G was monitored by immunoblot. Protein content load was monitored with anti-Lamin A. The results are representative of two independent experiments and are expressed as ratio of AIF or Endo G/Lamin A. C DNA fragmentation was analyzed by TUNEL (BrdU-FITC), the results are representative of a FACS plot of TUNEL. D Quantification of DNA fragmentation in C, histogram shows mean fluorescence intensity (MFI) fold change of M. bovis-infected and CsA-treated macrophages with respect to uninfected cells. The results are representative of three independent experiments and are expressed as total cell counts versus FITC signal in log10 scale. One-way ANOVA shows significant variation in infected cells versus CsA-treated uninfected control (p < 0.05) but not between CsA and negative control (p = 0.16536).