AIF and Endo G are translocated to macrophage nuclei as a consequence of M. bovis infection. Bovine macrophages were infected (MOI 10:1) with M. bovis AN5 strain at different time points (4, 8 and 16 h). Nuclear cell extracts from infected macrophages were analyzed by immunoblot, A AIF, B Endo G and C PARP-1. Protein content load was monitored with anti-Lamin A for nuclear extracts and anti-Tubulin for whole crude protein extracts. The results are representative of three independent experiments and are expressed as ratio of AIF and Endo G/Lamin A or PARP-1/Tubulin. Asterisk represents a p value equal or less than 0.05 of statistical significance.