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Figure 2 | Veterinary Research

Figure 2

From: Endonuclease G takes part in AIF-mediated caspase-independent apoptosis in Mycobacterium bovis-infected bovine macrophages

Figure 2

AIF and Endo G are translocated to macrophage nuclei as a consequence of M. bovis infection. Bovine macrophages were infected (MOI 10:1) with M. bovis AN5 strain at different time points (4, 8 and 16 h). Nuclear cell extracts from infected macrophages were analyzed by immunoblot, A AIF, B Endo G and C PARP-1. Protein content load was monitored with anti-Lamin A for nuclear extracts and anti-Tubulin for whole crude protein extracts. The results are representative of three independent experiments and are expressed as ratio of AIF and Endo G/Lamin A or PARP-1/Tubulin. Asterisk represents a p value equal or less than 0.05 of statistical significance.

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