Host factors determine the evolution of infection with Staphylococcus aureus to gangrenous mastitis in goats

Table 2 Primers used for the qPCR carried out with the RNA extracted from MFG

Genes	Primers	Sequence 5′–3′	Amplicon (bp)
Ccl5	Forward Reverse	ACC AGC AGC AAG TGC TCC AT CGC ACA CCT GAC GGT TCT T	86
Cd68	Forward Reverse	GAT CTG CTC TCC CTG AAG CTA CA CAT TGG GAC AAG AGA AAC TTG GT	79
Csn1s2	Forward Reverse	CTG GTT ATG GTT GGA CTG GAA AA AAC ATG CTG GTT GTA TGA AGT AAA GTG	76
Il17a	Forward Reverse	GCC CAC CTA CTG AGG ACA AG GCT GGA TGG TGA CAG AGT TC	246
Il17f	Forward Reverse	CACTCTGGAGGACCACATTG GAGTTCAGGGTCCTGTCTTC	216
Il1a	Forward Reverse	CTG AAG AAG AGA CGG TTG AG ATG CAT TCC TGG TGG ATG AC	164
Il1b	Forward Reverse	GAC AAC AAG ATT CCT GTG GCC TCT ACT TCC TCC AGA TGA AGT GT	101
Il8	Forward Reverse	TGA GAG TGG GCC ACA CTG C CAC AAC CTT CTG CAC CCA CTT	103
MIF	Forward Reverse	GCG CCT GCG ATT AGC CGC GTT CAT GTC GCA GAA	55
PTX3	Forward Reverse	CCG AGC TGT GCA GGG CT GCA CGC TTG CAA AAA TCT TCT T	101
PPIA	Forward Reverse	TGA CTT CAC ACG CCA TAA TGG T CAT CAT CAA ATT TCT CGC CAT AGA	62
RPS24	Forward Reverse	TGG TGGTGG CAA GAC AAC TG TTC TTC GCG TAA TCC AAG GAA	66

Gene names: C–C motif chemokine ligand 5 (CCL5); Alpha-S2-casein (CSN1S2); Macrophage migration inhibitory factor (MIF); Pentraxin 3 (PTX3); Peptidylprolyl isomerase A (PPIA); Ribosomal protein S24 (RPS24).

ISSN: 1297-9716