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Figure 5 | Veterinary Research

Figure 5

From: Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection

Figure 5

Far Western analysis of rTsSP binding to IEC protein. A SDS-PAGE of the IEC proteins. Lane M: the protein molecular weight marker; lane 1: the IEC lysate proteins. B Far-Western analysis of IEC proteins binding to rTsSP. The IEC protein was first reacted with rTsSP (lanes 1–3), IIL ES proteins (lanes 4–6) or PBS (lanes 7–9), and then recognized using anti-rTsSP serum (lanes 1, 4 and 7), infection serum (lanes 2, 5 and 8) or pre-immune serum (lanes 3, 6 and 9). C Far Western analysis of C2C12 protein binding to rTsSP. The C2C12 protein (lanes 1–3) was reacted first with rTsSP, and subsequently incubated with anti-rTsSP serum (lane 1), infection serum (lane 2) or pre-immune serum (lane 3). There was no binding between rTsSP and C2C12 protein.

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