Immunolocalization of TsSP by IFT with cryosections of T. spiralis ML and AW. A, B Cryosections of the ML were probed with anti-rTsSP serum, and immunostaining was observed at the cuticle and stichosome of ML. C Cryosections of the 6 days old female adults were recognized by anti-rTsSP serum and the immunostaining was located in embryos of the females. D The ML recognized by mouse infection serum as a positive control. The ML not recognized by pre-immune serum (E) and PBS (F) as negative controls. Scale-bars: 100 μm.