Identification of the infectivity of T. spiralis ML treated by ADCC assay. Worm burden of intestinal adults (A) and muscle larvae (B) was assessed in mice inoculated orally with T. spiralis ML treated by ADCC assay. The ML were incubated with 1:100 dilutions of anti-rTsSP serum and 2 × 105 mouse peritoneal macrophages (PM) at 37 °C for 24 h. Three groups of mice were orally inoculated with 100 ML treated by ADCC assay. The results are shown as the mean ± SD (n = 6). Asterisks demonstrate a remarkable difference (P < 0.01) in parasite burdens of the anti-rTsSP serum group relative to those in the normal serum group.