Figure 9

CRCoV (A) does not co-localize with clathrin in opposite to Alexa Fluor 488 conjugated transferrin (B). Cells treated with virus or transferrin were synchronized on ice for 60 min and incubated at 37 °C for 2, 5 or 20 min before they were washed and fixed. Clathrin HC are presented in red while stained virus nucleocapsid protein in green. C Negative controls for staining. D Co-localization changes in time. Scale bar 10 µm.