Figure 6

siRNA treatment. HRT-18G cells were transfected twice using scrambled negative control siRNA or caveolin-1 siRNA, infected, synchronized on ice for 60 min and incubated at 37  °C for 60 min before they were washed and fixed. CRCoV virions are presented in green, blue denotes DNA and red represents caveolin 1 (A) or actin (B), each image is a single confocal plane. C Negative control for staining. Bar 10 µm D Western blot signal of caveolin-1 and GAPDH expression in transfected cells. ctrl: control, sh: sham transfected cells, scRNA: scrambled RNA, siRNA: caveolin-1 siRNA. Scale bar 10 µm.