Figure 3

Effect of furin inhibitor—decanoyl-RVKR-chloromethyl ketone (CMK) on CRCoV replication (A) and cell to cell spread (B). A Graph shows number of virus positive cells at 4^th day pi normalized to control. HRT-18G cells were pretreated with inhibitor before infection and it was present post-infection. B 3 h pi cells were overlaid with CMK containing medium and were propagated in this conditions until fixation at 4^th day pi. CRCoV virions are presented in green, blue denotes DNA and red represents actin. Scale bar 20 µM.