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Figure 2 | Veterinary Research

Figure 2

From: Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells

Figure 2

Co-localization of CRCoV with early endosome marker EEA1. Virus treated cells were synchronized on ice for 60 min and incubated at 37  °C before they were washed and fixed with 4% paraformaldehyde in PBS. Localization was analyzed by confocal microscopy after performing a double immunofluorescence staining to visualize virus nucleocapsid (left) and early endosomes (middle). Right panel shows merged image with virus presented as green and EEA1 as red. Cell nuclei are shown in blue. Control—negative control, cells incubated with mock sample (green) and stained with isotype antibodies (red). Scale bar 10 µm. Graph presents co-localization change in time.

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