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Table 1 Description and sequences of the oligonucleotides designed to quantify specific ovine genes using real-time PCR

From: Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection

Genes Sequences Amplified product size (bp) Accession number
Foxp3 F 5′-GCCCATCTGGCTGGGAAGATGGCCCAAACC-3′ 166 NM_001144947.1
R 5′-AGAGGTGCCTCCGCACGGCAAACAGG-3′
IL10 F 5′-TCAGCCGTGCTCTGTTGCCTGGTCTTCC-3′ 124 NM_001009327.1 
R 5′-GGACGTCCCGCAGCATGTGGGGCAG-3′
TGFβ F 5′-GGGCTTTCGCCTCAGTGCCCACTGTTC-3′ 151 NM_001009400.1
R 5′-CAGAGGGGTGGCCATGAGGAGCAGG-3′
TNFα F 5′-CCACGCTCTTCTGCCTGCTGCACTTCGG-3′ 146 NM_001024860.1
R 5′-AACGTGGGCTACCGGCTTGTTATTTGAGGC-3′
IL1β F 5′-GAAGCTGAGGAGCCGTGCCTACGAACA-3′ 185 NM_001009465.2
R 5′-CCAGCACCAGGGATTTTTGCTCTCTGTCC-3′