Table 1 Description and sequences of the oligonucleotides designed to quantify specific ovine genes using real-time PCR
Genes | Sequences | Amplified product size (bp) | Accession number |
|---|---|---|---|
Foxp3 | F 5′-GCCCATCTGGCTGGGAAGATGGCCCAAACC-3′ | 166 | NM_001144947.1 |
R 5′-AGAGGTGCCTCCGCACGGCAAACAGG-3′ | |||
IL10 | F 5′-TCAGCCGTGCTCTGTTGCCTGGTCTTCC-3′ | 124 | NM_001009327.1 |
R 5′-GGACGTCCCGCAGCATGTGGGGCAG-3′ | |||
TGFβ | F 5′-GGGCTTTCGCCTCAGTGCCCACTGTTC-3′ | 151 | NM_001009400.1 |
R 5′-CAGAGGGGTGGCCATGAGGAGCAGG-3′ | |||
TNFα | F 5′-CCACGCTCTTCTGCCTGCTGCACTTCGG-3′ | 146 | NM_001024860.1 |
R 5′-AACGTGGGCTACCGGCTTGTTATTTGAGGC-3′ | |||
IL1β | F 5′-GAAGCTGAGGAGCCGTGCCTACGAACA-3′ | 185 | NM_001009465.2 |
R 5′-CCAGCACCAGGGATTTTTGCTCTCTGTCC-3′ |