Gene expression of Foxp3, IL-10, TGF-β, IL-1β, and TNF-α. Each bar represents the mean ± SD of the mRNA molecules/ng of total RNA quantified individually in each of the five animals per experimental condition and sampling time, after three real-time PCR reactions per individual. a for comparison of each treatment with the negative control, and b for comparisons between the infected and infected and immunized groups. The levels of gene expression were increasing gradually throughout the experiment in the transcription factor, foxp3 and two regulatory cytokines studied (IL-10, TGF-β), reaching its higher expression at 18 dpi, and for IL-10 also markedly at 3 and 9 dpi, coinciding with a slight increase in the expression of Foxp3. Foxp3, IL-10, and TGF-β showed significant differences in comparison with the negative control group at 1, 3, and 9 dpi, respectively. At the same time, differences were shown between immunized and infected groups at 1 and 3 dpi in Foxp3 and 3 dpi in IL-10. The expression of proinflammatory cytokines (IL-1β and TNF-α) was gradually increasing along the experience, showing the typical expression of an acute stage of disease.