Skip to main content

Table 1 MAbs used in the present study

From: A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria

mAb Isotype Specificity/source Fluorochrome Labeling
ILA11A IgG2a CD4 WSUMAC PE CY5.5 Indirect
CACT138A IgG1 CD4 WSUMAC Alexa Fluor® 647 Indirect
7C2B IgG2a CD8 WSUMAC PE CY5.5 Indirect
CACT80C IgG1 CD8 WSUMAC Alexa Fluor® 647 Indirect
ILA116A IgG3 CD45R0 WSUMAC Alexa Fluor® 488; PE Indirect
CAM36A IgG1 CD14 WSUMAC Alexa Fluor® 647 Indirect
209MD26A IgG2a CD209 WSUMAC PE CY5.5 Indirect
δG9 IgG2b Perforin Biolegend PE Direct
B-D48 IgG1 Perforin Biolegend PE/CY7 Direct
351927 IgG2a Granzyme B R&D Systems PE Direct