Flow cytometric analysis showing expression of newly synthesized perforin, GnzB in CD4, CD8 T cells. A Newly synthesized perforin was highly expressed in CD8 T cells from unvaccinated steers with low expression in CD4 T cells (right set of graphs). Newly synthesized perforin was below the limits of detection in primed mdPBMC incubated with uninfected MoMΦ (center set of graphs). B Expression of GnzB in infected MoMΦ was low in CD4 T cells. GnzB was present in approximately half of the CD8 T cells.