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Figure 4 | Veterinary Research

Figure 4

From: A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria

Figure 4

Two-way ANOVA comparison CD4, CD8 T cell response unvaccinated and vaccinated steers to Ag-pulsed MoDC. The factors were steer-status (three levels: S1-unvaccinated, S2-vaccinated, S3-vaccinated) and treatments (four levels: D0, Control, ∆Map/relA, MMP). Tukey HSD (overall α = 0.05) was applied post-hoc to determine the statistical significance of comparisons of interest. For each T cell type (CD4 and CD8), the mean and standard deviation for each treatment effect (n = 6 independent experiments) within each steer-status is shown. The two-way ANOVAs for CD4 and CD8 T cells were significant (F CD4  = 158.2463, F CD8  = 379.0110; each, P < 0.0001) and each included a significant interaction effect between treatments and steer-status (F CD4  = 56.3527, F CD8  = ;165.4418; each, P < 0.0001). For both CD4 and CD8 T cells, the mean effects of ∆Map/relA and MMP in vaccinated steer S3 were greater than in unvaccinated steers S1 and S2 (each, P < 0.0001); significant differences not detected between unvaccinated steers in the mean effects of ∆Map/relA (P CD4  = 1.0000, P CD8  = 1.0000) or MMP (P CD4  = 0.9997, P CD8  = 1.0000).

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Veterinary Research

ISSN: 1297-9716