Ovotransferrin stability in faeces from birds. The ovotransferrin stability in the faeces was assessed by spiking two independent faeces pools from healthy birds with ovotransferrin at a final concentration of 200 µg/g. A The spiked samples were aliquoted and incubated at room temperature for 0, 1, 2, 6 or 24 h after which the ovotransferrin concentration was measured by ELISA. B The ovotransferrin stability in the faeces with (black) or without (grey) the addition of protease inhibitors was assessed after 0, 2 or 24 h incubation at room temperature. The % ovotransferrin recovery was calculated relative to the 0 h timepoint.