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Table 2 Differentially expressed (DE) genes identified in Mycoplasma suis -infected pigs tested by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR)

From: RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

 

Gene ID

Gene symbol

Description

qRT-PCR FC

RNA-Seq FCa

qRT-PCR p-value

RNA-Seq p-value**

DE up-regulated genes

100145895

ISG15

ISG15 ubiquitin-like modifier

2.79

4.72

0.07

0.04

DE down-regulated genes

100513324

IL22RA2

Interleukin 22 receptor, alpha 2

0.01

0.02

0.03

0.01

 

574058

CD274

CD274 molecule

0.11

0.17

0.01

0.01

 

100514901

BCL2L14

BCL2-like 14 (apoptosis facilitator)

0.24

0.25

0.01

0.07

 

414371

CCR5

chemokine (C–C motif) receptor 5

0.37

0.33

0.04

0.03

 

397644

CD180

CD180 molecule

0.37

0.26

0.01

0.02

 

397271

AIF1

Allograft inflammatory factor 1

0.19

0.20

0.00

0.01

 

397683

IL15

Interleukin 15

0.22

0.24

0.03

0.02

 

100524563

PTPRO

Protein tyrosine phosphatase, receptor type, O

0.40

0.28

0.01

0.01

 

397384

TLR8

Toll-like receptor 8

0.40

0.32

0.04

0.05

  1. FC: fold change of a DE gene in the infected group related to the control group, GAPDH was used as reference control gene for calculation of relative quantification.
  2. ** p-values based on Cufflinks results. Note that a FC of 0.01 of a down-regulated gene indicates that infected group has 1/100 the amount of target RNA as the control group.
  3. aFC based on Cufflinks results.