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Table 2 Differentially expressed (DE) genes identified in Mycoplasma suis -infected pigs tested by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR)

From: RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

  Gene ID Gene symbol Description qRT-PCR FC RNA-Seq FCa qRT-PCR p-value RNA-Seq p-value**
DE up-regulated genes 100145895 ISG15 ISG15 ubiquitin-like modifier 2.79 4.72 0.07 0.04
DE down-regulated genes 100513324 IL22RA2 Interleukin 22 receptor, alpha 2 0.01 0.02 0.03 0.01
  574058 CD274 CD274 molecule 0.11 0.17 0.01 0.01
  100514901 BCL2L14 BCL2-like 14 (apoptosis facilitator) 0.24 0.25 0.01 0.07
  414371 CCR5 chemokine (C–C motif) receptor 5 0.37 0.33 0.04 0.03
  397644 CD180 CD180 molecule 0.37 0.26 0.01 0.02
  397271 AIF1 Allograft inflammatory factor 1 0.19 0.20 0.00 0.01
  397683 IL15 Interleukin 15 0.22 0.24 0.03 0.02
  100524563 PTPRO Protein tyrosine phosphatase, receptor type, O 0.40 0.28 0.01 0.01
  397384 TLR8 Toll-like receptor 8 0.40 0.32 0.04 0.05
  1. FC: fold change of a DE gene in the infected group related to the control group, GAPDH was used as reference control gene for calculation of relative quantification.
  2. ** p-values based on Cufflinks results. Note that a FC of 0.01 of a down-regulated gene indicates that infected group has 1/100 the amount of target RNA as the control group.
  3. aFC based on Cufflinks results.