RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

Table 1 Primers used for validation of differentially expressed (DE) genes in Mycoplasma suis -infected pigs by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR)

	Gene symbol	Gene description	Primer forward (5′–3′)	Primer reverse (5′–3′)	Amplicon length (bp)
Reference gene	GAPDH		TTGGCTACAGCAACAGGGTG	CAGGAGATGCTCGGTGTGTT	166
DE genes	ISG15	ISG15 ubiquitin-like modifier	ATGTGCTTCAGGATGGGGTG	AGGATGCTCAGTGGGTCTCT	100
	IL22RA2	Interleukin 22 receptor, alpha 2	ACCAGCAACAGCAGCATCTA	TGAGTGCATCCCAGCCAAG	177
	CD274	CD274 molecule	TTACCCAGAGGCCGAAGTCA	TCCTCTCTCTGGGAACTGGT	87
	BCL2L14	BCL2-like 14 (apoptosis facilitator)	TGCCTACAGGGTTCCGTTTC	TTCGTGGTCTAAGCGCTGTT	113
	CCR5	Chemokine (C-C motif) receptor 5	CAGTGGGTCTAACAGGCTGG	GCGTCTGACGATGTGCTTTC	151
	CD180	CD180 molecule	TGCTCATCGTCCTGCTCATTT	CCAGCTCCAGGAACCAATCT	178
	AIF1	Allograft inflammatory factor 1	GCGAGAGAACAGGAAAAGCC	AGCCCCTTCAATTCCACCAC	100
	IL15	Interleukin 15	GCTCATCCCAATTGCAAAGT	TTCCTCCAGCTCCTCACATT	189 [37]
	PTPRO	Protein tyrosine phosphatase, receptor type, O	AAGCACCAGGACGACTTAGC	AACCCCAAAACTCAGCCCAA	174
	TLR8	Toll-like receptor 8	CGGAAGGCTTGTTTTGGCAA	CGACCAAACATCACCGAGGA	112

ISSN: 1297-9716