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Table 2 Verification of real-time PCR for some differentially expressed genes

From: Comparative liver transcriptome analysis in ducklings infected with duck hepatitis A virus 3 (DHAV-3) at 12 and 48 hours post-infection through RNA-seq

Genes Transcriptomics fold change (infected/control) qRT-PCR fold change (2−∆∆Ct) (infected/control) Gene function
RIG-1 21.3 6.69 RIG-like receptor
MDA5 37.3 31.48 RIG-like receptor
TLR7 6.429 25.3 Toll-like receptor
TLR3 4.564 4.23 Toll-like receptor
IFN-α Inf 160.9 Type I IFN
IFN-β 226.29 702.86 Type I IFN
IFN-γ 7.66 5.63 Type II IFN
IL-1β 78.09 Proinflammatory cytokines
IL-2 Inf 171.88 Proinflammatory cytokines
IL-6 31.16 68.62 Proinflammatory cytokines
MX 1464 3880.75 IFN-stimulated genes
OAS 442.74 191.77 IFN-stimulated genes
PKR 31.25 19.85 IFN-stimulated genes
IFIT5 4110.1 2103.33 IFN-stimulated genes
  1. “Inf” represented the differential expression is infinite is in the results of transcriptome sequencing.
  2. “–” represented the gene did not detect through RNAseq.