Pathological changes in the livers of DHAV-3-infected ducklings at different time points. A Histopathological examination of duckling livers in the control group. B Histopathological changes were not observed in the liver at 12 hpi. C Many cells have dissolved and disappeared, hepatocyte necrosis with hemorrhage at 48 hpi. D DHAV-3 replication in the livers of ducklings with DHAV-3 infection at 12, 48, and 72 hpi. The data are expressed as mean ± standard deviation. Three dead ducklings were selected for detecting the viral RNA load using the qRT-PCR method.