Table 1 Primary antibodies used in flow cytometric analysis of this paper
Target | Isotype | Clone | Source (reference) | Fluorochrome | Conjugation or labeling |
|---|---|---|---|---|---|
Flow cytometric analysis of PD-1 and LAG-3 | |||||
 CD4 | Mouse IgG2a | CC8 | Bio-Rad | FITC | (Conjugated primary antibody) |
 CD8 | Mouse IgG2a | CC63 | Bio-Rad | FITC | (Conjugated primary antibody) |
 CD3 | Mouse IgG1 | MM1A | WSU monoclonal Antibody Center | PerCp/Cy5.5 | Lightning-Link PerCp/Cy5.5 Conjugation Kit (Innova Biosciences) |
 IgM | Mouse IgG1 | IL-A30 | Bio-Rad | PE/Cy7 | Lightning-Link PE/Cy7 Conjugation Kit (Innova Biosciences) |
 TCR1-N24 (δ chain) | Mouse IgG2b | GB21A | WSU monoclonal Antibody Center | APC/Cy7 | Lightning-Link APC/Cy7 Conjugation Kit (Innova Biosciences) |
 PD-1 | Rat IgG2a | 5D2 | In house [13] | APC | APC-conjugated anti-rat Ig antibody (Southern Biotech) |
 LAG-3 | Rat IgG1 | 71-2D8 | In house [21] | PE | PE-conjugated anti-rat IgG1 antibody (Southern Biotech) |
Flow cytometric analysis of IFN-γ/TNF-α-producing T cells (intracellular staining) | |||||
 CD4 | Mouse IgG2a | CC8 | Bio-Rad | FITC | (Conjugated primary antibody) |
 CD8 | Mouse IgG2a | CC63 | Bio-Rad | PerCp/Cy5.5 | Lightning-Link PerCp/Cy5.5 Conjugation Kit (Innova Biosciences) |
 TCR1-N24 (δ chain) | Mouse IgG2b | GB21A | WSU monoclonal Antibody Center | Alexa Fluor 647 | Zenon Alexa Fluor 647 Mouse IgG2b Labeling Kit (Thermo Fisher Scientific) |
 IFN-γ | Mouse IgG1 | CC302 | Bio-Rad | PE | (Conjugated primary antibody) |
 TNF-α | Mouse IgG2b | CC328 | Bio-Rad | APC/eFluor780 | Streptavidin APC/eFluor780 (eBioscience) |