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Table 1 Primary antibodies used in flow cytometric analysis of this paper

From: Cooperation of PD-1 and LAG-3 in the exhaustion of CD4+ and CD8+ T cells during bovine leukemia virus infection

Target Isotype Clone Source (reference) Fluorochrome Conjugation or labeling
Flow cytometric analysis of PD-1 and LAG-3
 CD4 Mouse IgG2a CC8 Bio-Rad FITC (Conjugated primary antibody)
 CD8 Mouse IgG2a CC63 Bio-Rad FITC (Conjugated primary antibody)
 CD3 Mouse IgG1 MM1A WSU monoclonal Antibody Center PerCp/Cy5.5 Lightning-Link PerCp/Cy5.5 Conjugation Kit (Innova Biosciences)
 IgM Mouse IgG1 IL-A30 Bio-Rad PE/Cy7 Lightning-Link PE/Cy7 Conjugation Kit (Innova Biosciences)
 TCR1-N24 (δ chain) Mouse IgG2b GB21A WSU monoclonal Antibody Center APC/Cy7 Lightning-Link APC/Cy7 Conjugation Kit (Innova Biosciences)
 PD-1 Rat IgG2a 5D2 In house [13] APC APC-conjugated anti-rat Ig antibody (Southern Biotech)
 LAG-3 Rat IgG1 71-2D8 In house [21] PE PE-conjugated anti-rat IgG1 antibody (Southern Biotech)
Flow cytometric analysis of IFN-γ/TNF-α-producing T cells (intracellular staining)
 CD4 Mouse IgG2a CC8 Bio-Rad FITC (Conjugated primary antibody)
 CD8 Mouse IgG2a CC63 Bio-Rad PerCp/Cy5.5 Lightning-Link PerCp/Cy5.5 Conjugation Kit (Innova Biosciences)
 TCR1-N24 (δ chain) Mouse IgG2b GB21A WSU monoclonal Antibody Center Alexa Fluor 647 Zenon Alexa Fluor 647 Mouse IgG2b Labeling Kit (Thermo Fisher Scientific)
 IFN-γ Mouse IgG1 CC302 Bio-Rad PE (Conjugated primary antibody)
 TNF-α Mouse IgG2b CC328 Bio-Rad APC/eFluor780 Streptavidin APC/eFluor780 (eBioscience)
  1. FITC: fluorescein isothiocyanate, PerCp: peridinin-chlorophyll-protein complex, Cy: cyanin, PE: phycoerythrin, APC: allophycocyanin.