Table 3 Values of each evaluated technique corresponding to a unit of a particular method (first column) in each M. hyopneumoniae strain culture

ATP (pmol ATP/mL)

J

–

1.76E+05

6.09E+05

6.40E+04

11

–

4.05E+05

1.23E+06

2.09E+05

232

–

1.66E+03

1.08E+06

1.71E+05

Mean (± SD)

–

1.94E+05 (± 2.02E+05)

9.72E+05 (± 3.23E+05)

1.48E+05 (± 7.51E+04)

CCU (CCU/mL)

J

2.10E−04

–

9.68E+02

3.79E+00

11

3.92E−05

–

1.02E+03

4.65E+00

232

1.23E−02

–

7.79E+04

3.77E+03

Mean (± SD)

4.17E−03 (± 7.00E−03)

–

2.66E+04 (± 4.44E+04)

1.26E+03 (± 2.17E+03)

Fluorimetry (M. hyopneumoniae copies/mL)

J

7.99E−08

< 1

–

< 1

11

6.47E−09

< 1

–

< 1

232

1.53E−09

< 1

–

< 1

Mean (± SD)

2.93E−08 (± 4.39E−08)

NA

–

NA

qPCR (M. hyopneumoniae copies/mL)

J

2.12E−04

< 1

3.50E+02

–

11

2.51E−05

< 1

3.69E+02

–

232

1.18E−05

<  1

3.20E+02

–

Mean (± SD)

8.30E−05 (± 1.12E−04)

NA

3.47E+02 (± 2.49E+01)

–