Skip to main content

Table 3 Values of each evaluated technique corresponding to a unit of a particular method (first column) in each M. hyopneumoniae strain culture

From: Assessment of the in vitro growing dynamics and kinetics of the non-pathogenic J and pathogenic 11 and 232 Mycoplasma hyopneumoniae strains

Technique (one unit) Strain Technique (equivalence units)
ATP (pmol ATP/mL) CCU (CCU/mL) Fluorimetry (M. hyopneumoniae copies/mL) qPCR (M. hyopneumoniae copies/mL)
ATP (pmol ATP/mL) J 1.76E+05 6.09E+05 6.40E+04
11 4.05E+05 1.23E+06 2.09E+05
232 1.66E+03 1.08E+06 1.71E+05
Mean (± SD) 1.94E+05 (± 2.02E+05) 9.72E+05 (± 3.23E+05) 1.48E+05 (± 7.51E+04)
CCU (CCU/mL) J 2.10E−04 9.68E+02 3.79E+00
11 3.92E−05 1.02E+03 4.65E+00
232 1.23E−02 7.79E+04 3.77E+03
Mean (± SD) 4.17E−03 (± 7.00E−03) 2.66E+04 (± 4.44E+04) 1.26E+03 (± 2.17E+03)
Fluorimetry (M. hyopneumoniae copies/mL) J 7.99E−08 < 1 < 1
11 6.47E−09 < 1 < 1
232 1.53E−09 < 1 < 1
Mean (± SD) 2.93E−08 (± 4.39E−08) NA NA
qPCR (M. hyopneumoniae copies/mL) J 2.12E−04 < 1 3.50E+02
11 2.51E−05 < 1 3.69E+02
232 1.18E−05 <  1 3.20E+02
Mean (± SD) 8.30E−05 (± 1.12E−04) NA 3.47E+02 (± 2.49E+01)
  1. Equivalences were obtained by fitting the linear regression models ofeach pair of techniques comparison to data from the maximal culture growth (log phase).