Figure 3
Systemic IFNγ response after N. caninum challenge in pregnant ewes. IFNγ response in intravenously challenged animals (A) and in IV and SC challenged animals with 104 Nc-Spain7 tachyzoites (B). Each point represents the mean + SD at the different sampling times for each group. IFNγ levels from G1 on day 10 pi are not available due to problems in antigen stimulation assays of the peripheral blood. Data beyond day 28 pi are not included because several animals did not maintain pregnancy and were therefore sacrificed. Concentrations of IFNγ are expressed in pg/mL. The cellular immune response represented in figure was analysed using two-way ANOVA of repeated measures. For significant differences between infected groups, (***) indicates P < 0.001, and (****) indicates P < 0.0001.