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Table 1 Differentially expressed structural and extracellular matrix proteins of common carp

From: Quantitative shotgun proteomics distinguishes wound-healing biomarker signatures in common carp skin mucus in response to Ichthyophthirius multifiliis

NCBI accession number

Protein

Number of quantified peptides

BLASTp results

1 dpe

9 dpe

Function

Structural and extracellular matrix proteins

 KTG36050.1

cypCar_00022254 [Cyprinus carpio]

6

collagen alpha-3(VI) chain-like isoform X3 [Salmo salar], XP_014019344.1, 62% identity

−18.7*

−37.1*

Matrix component organisation

 KTF73577.1

cypCar_00045321, partial [Cyprinus carpio]

6

collagen alpha-2(I) chain-like [Sinocyclocheilus rhinocerous], XP_016385859.1, 90% identity

−50.2*

−35.7*

Matrix component organisation

 XP_018968199.1

collagen alpha-1(I) chain-like [Cyprinus carpio]

5

–

−11.3*

−9.9*

Matrix component organisation

 XP_018967439.1

collagen alpha-2(VI) chain-like [Cyprinus carpio]

3

–

−6.0*

−4.5*

Matrix component organisation

 KTF78707.1

cypCar_00043888, partial [Cyprinus carpio]

4

–

−99.9*

−88.2*

Matrix component organisation

 XP_018967802.1

collagen alpha-1(VI) chain-like [Cyprinus carpio]

5

–

−6.1*

−8.3*

Matrix component organisation

 XP_018962933.1

collagen alpha-1(XIV) chain-like [Cyprinus carpio]

2

–

−27.3*

−31.2*

Matrix component organisation

 KTF76685.1

cypCar_00016174 [Cyprinus carpio]

2

collagen alpha-2(VI) chain-like [Cyprinus carpio], XP_018932781.1, 96% identity

−3.7*

−5.3*

Matrix component organisation

 XP_018933497.1

collagen alpha-1(XIV) chain-like [Cyprinus carpio]

2

–

−5.1*

−5.2*

Matrix component organisation

 XP_018950001.1

dermatopontin-like [Cyprinus carpio]

2

–

−4.9*

−10.9*

Matrix component organisation

 XP_018958339.1

src substrate cortactin-like [Cyprinus carpio]

2

–

−1.2

2.6

Actin regulatory protein, stabilises actin filaments

 KTF82439.1

cypCar_00015496 [Cyprinus carpio]

2

lumican-like [Sinocyclocheilus rhinocerous], XP_016414194.1, 96% identity

−8.7*

−9.1*

Binds collagen fibrils and regulates its structure, and enhances macrophages and neutrophils recruitment

 P_018921152.1

keratin, type I cytoskeletal 18 [Cyprinus carpio]

6

–

2.0

11.4*

Protect cells from mechanical and non mechanical injuries, pore-forming activities

 XP_018948461.1

keratin, type I cytoskeletal 18-like [Cyprinus carpio]

3

–

1.2

−3.5*

Protect cells from mechanical and non mechanical injuries, pore-forming activities

 XP_018980214.1

MYH16 isoform X1 [Cyprinus carpio]

6

myosin-11-like [Sinocyclocheilus rhinocerous]

Sequence ID: XP_016405427.1, 90% identity

4.4*

2.5

Transendothelial migration of leukocytes

 XP_018930066.1

LOC109057295 [Cyprinus carpio]

6

myosin heavy chain, clone 203 [Danio rerio], XP_009289654.1, 48% identity

−1.4

6*

Transendothelial migration of leukocytes

 KTF75732.1

cypCar_00036737 [Cyprinus carpio]

5

PREDICTED: papilin-like [Sinocyclocheilus anshuiensis]

XP_016354784.1, 92% identity

−15*

−11.4*

metalloproteinase inhibitor, tissue rearrangement

 KTF72113.1

cypCar_00043727, partial [Cyprinus carpio]

2

PREDICTED: neoverrucotoxin subunit beta-like [Sinocyclocheilus anshuiensis], XP_016330044.1, 91% identity

−1

3.5*

Microtubule organization and stabilization, pore-forming activities

 XP_018924262.1

olfactomedin-4-like isoform X2 [Cyprinus carpio]

4

–

3.3*

1.3

Negative feedback effect on NF-κB activation

  1. Fold change (infected vs control) was statistically analyzed in Ichthyophthirius multifiliis exposed infected common carp at 1 and 9 days post-exposure (dpe). * Denotes values (italic) statistically significant according to both ANOVA with FDR-adjusted p value < 0.001 and fold change < −3 or > +3.