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Table 2 Primers used in this study

From: The putative amino acid ABC transporter substrate-binding protein AapJ2 is necessary for Brucella virulence at the early stage of infection in a mouse model

Primers Sequences
27735-UF GCTCTAGAGCTATCATGGCACCACGCAGGAAC (Xba I underlined)
27735-UR GGCGGGGCGTACATCAGCCCCAGCAACGCACCCCAAACTG
27735-DF CAGTTTGGGGTGCGTTGCTGGGGCTGATGTACGCCCCGCC
27735-DR GCTCTAGAGCCTTTTCAATGAATGCACCGGC (Xba I underlined)
P1 CTGGGAGGAGGAACAATGAA
P2 CAGCCTGCTCAAGATCAACC
P3 GCGAAGGCGGAGCAATCT
RT-18500-F CTGGTTGCTGGACTTCGTGT
RT-18500-R GCTTGCCACGTCTTTCGATG
RT-20725-F AGCAATCTCAAGGCAACGGA
RT-20725-R GCATGCGAGATGGACGAAAC
RT-30275-F AGTCGACATGTCTTCGCTGG
RT-30275-R GATCAGGTCATGTCCCACGG
RT-30280-F AGGCGTAACGGATGTCTTGG
RT-30280-R CTGCGCGCTGAAAGAGATTG
RT-26600-F ACCATGCTTGATTCGCTTGC
RT-26600-R GTCAACTGATTGCGATGGGC
RT-26930-F AAGCCTGCAAGACCTCAACC
RT-26930-R CTGAAGCTTTGGGTCTGCCT
RT-31530-F GCCGCTCAACGAAATCATCC
RT-31530-R GTCACGATGCGCTGAAGAAC
RT-27100-F CTCGGCATGCAGAACAATCC
RT-27100-R TCGCGGTCCGTCTTGTTATC
RT-31650-F GATTCCACACGGCCAACAAC
RT-31650-R GTGGCGTTGAACATGAGCTG
RT-29200-F GACGGGGCGGAGATTGTTTA
RT-29200-R CGCAGTTCGGTTTGTTCCAG
RT-28255-F ATGACCTATGCGGTTCAGGC
RT-28255-R CTCGATCTTTGGGCGGAACA
RT-18900-F CGGCAACACCTTTCAACTCC
RT-18900-R CCTTTCGTCCACGCAGATCA
RT-30140-F CCAGCTTCATGTCGCTTCCT
RT-30140-R AGAATAAGCTGGTCGCGGAA
RT-18875-F CTGGAGTACGGCCAGTGAAG
RT-18875-R ATCAGGTTGACGAGCCTGTG
RT-27050-F ACAGCAACATCATGCGGGTA
RT-27050-R ATATGCTGGTCGTTCAGGGC
RT-23095-F ACCAGATCAATCTCGTGGGC
RT-23095-R GCATGAACGCGAAAAACCCT
RT-31525-F AACAGGTCGAATCACGGCTC
RT-31525-R ATCCGATGTTTTGCGGCTGA
RT-22085-F AAAATTGCCGAACAGGCCAC
RT-22085-R GGCCTTGTTGCCGTATTCAC
RT-27055-F ACGGTTATGGCTTCCGGTTC
RT-27055-R ATCTTGACGGTTGCTCCCAG
RT-GAPDH-F GACATTCAGGTCGTCGCCATCA
RT-GAPDH-R TCTTCCTTCCACGGCAGTTCGG