Strains or plasmids | Descriptions | Sources or references |
---|---|---|
Bacterial strains | ||
B. abortus S2308 | Wild-type strain; Smooth phenotype | CVCC |
∆27735 | bab_RS27735 gene deletion mutant strain; Smooth phenotype | This study |
E. coli DH5α | F− φ80lacZ∆M15∆(lacZYA-argF)U169 recA1 endA1 hsdR17(r −k , m +k ) phoA supE44 thi-1 gyrA96 relA1 λ− | Invitrogen |
Plasmids | ||
pSC | AmpR; pUC19 plasmid containing SacB gene | [10] |