Table 1 Primers for amplifying the truncated fiber2 gene and the linear pcDNA3.1
PCR product | Primer sequence (5′–3′) |
|---|---|
F2_1-415aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTACTATAGCATAGAAG | |
F2_1-382aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTAGGCTGAACACTTGG | |
F2_1-349aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTACGTGAGGGTGGCGG | |
F2_1-316aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTATTGGCGGAGTTGAG | |
F2_1-283aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTAGGTAGTAGGCGCAA | |
F2_1-250aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTAGGAGACGCTCCCCC | |
F2_1-217aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTAGGTCCGCTGGGATC | |
F2_1-182aa | Forward: AGCTTGGTACCGAATGCTCCGGGCCCCTAA |
Reverse: ATATCTGCAGAATTTACCTGCGCTTTTAGG | |
Linear pcDNA3.1 | Forward: GAATTCTGCAGATATCCAGCACAGTG |
Reverse: GCTCGGTACCAAGCTTAAGTTTAAACG |