Figure 3

Infection kinetics of PRV and IHNV in A. salmon. A PRV established a persistent infection. Challenge with IHNV did not influence PRV relative viral loads measured in spleens. Marks represent the median value of Ct obtained for each group at each time point. B IHNV challenge resulted in acute infection peaking 9 days post-challenge followed by a rapid decrease of the viral loads in the spleen. Negligible levels of IHNV infection are observed in PRV previously challenged fish. Horizontal lines represent the median value of Ct obtained from three individuals for each group at each time point. C Perinuclear immunofluorescent staining of PRV σ1 antigen in Atlantic salmon erythrocytes infected with PRV (100×).