Figure 6

Detection of secreted IFNγ in plasma after in vitro re-stimulation. A Whole blood sampled from sheep either after last immunization (post-immunization), just before challenge (prechallenge) or 8 days after challenge (post-challenge) was incubated overnight with RVFV antigen. IFNγ levels in plasma were determined by capture ELISA. B Detection of virus specific T-cell responses by IFNγ ELISPOT in vaccinated and control sheep after last immunization, just before challenge or 8 days after challenge. Asterisks indicate the level of significance with respect to control values (*p ≤ 0.05).