Figure 2From: Efficacy of different DNA and MVA prime-boost vaccination regimens against a Rift Valley fever virus (RVFV) challenge in sheep 12 weeks following vaccinationViremia titers in vaccinated or mock vaccinated sheep upon RVFV challenge. Viremia was determined by direct virus titration of individual whole blood samples recovered from days 1–12 post-infection. Lysed blood samples (see “Materials and methods” section) were incubated for 6 days in Vero cells seeded in multi-well 96 plates. End point titers were estimated by the Reed and Muench method [25] based on observation of cytopathic effect upon cell staining.Back to article page