Table 1 Bacterial strains and plasmids used in this study

Strains

 BL21(DE3)pLysS

F-ompT hsdSB (rB- mB-) dcm galλ (DE3) pLysS Cmr

Promega

 JOL1682

E. coli BL21(DE3) pLysS expressing LfliC via pET28a (+) system

This study

Plasmids

 pET28(+)

IPTG-inducible, T7 expression vector, C-terminal 6×His tag, Kan^R

Novagen, USA

 pET28(+)-LfliC

pET28a derivative containing LfliC

This study