Figure 6

Assaying the poly (I:C) and CVP1 complexes by gel retardation electrophoresis and detection the relative mRNA levels of IFN-β by real-time PCR. A Testing the complex of poly (I:C) and CVP1 was conducted by electrophoresis and ethidium bromide staining. Lane 1: production of CVP1 peptide co-incubated with poly (I:C); Lane 2: poly (I:C) only; B The mRNA level of IFN-β obtained from the complexes of CVP1 peptide and TAT conjugated with poly (I:C), respectively, TAT and CVP1 peptide only, transfection reagent with poly (I:C) and blank cells. Single asterisk (*) and double asterisks (**) indicated P < 0.05 and P < 0.01, respectively.