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Figure 5 | Veterinary Research

Figure 5

From: A novel CAV derived cell-penetrating peptide efficiently delivers exogenous molecules through caveolae-mediated endocytosis

Figure 5

The efficiency of CVP1 peptide delivering DNA into HCT116 cells. A Detection of DNA and peptide complexes was conducted by electrophoresis and ethidium bromide staining. Lane 1: pCDNA3.1-RFP plasmid conjugated with CVP1 peptide; Lane 2: pCDNA3.1-RFP plasmid only; Fluorescence microscope images of the expression of the red fluorescence protein. The images obtained at a ×40 magnification. B Images of the CVP1/RFP complex, Mut-CVP1/RFP complex, TAT/RFP complex, pCDNA3.1-RFP only and transfection reagent lipofectamine 2000/RFP complex. C Quantification of the transfection efficiency using flow cytometry. Single asterisk (*) and double asterisks (**) indicated P < 0.05 and P < 0.01, respectively.

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