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Table 2 Summary of clean reads produced by the sRNA sequencing after filtering the contaminated reads

From: Identification and characterization of microRNA in the lung tissue of pigs with different susceptibilities to PCV2 infection

Type

LW-u

LW-i

YL-u

YL-i

Amount

Percent (%)

Amount

Percent (%)

Amount

Percent (%)

Amount

Percent (%)

Total_readsa

9 600 000

 

9 597 432

 

9 600 000

 

9 600 000

 

High_qualityb

9 573 498

100

9 570 066

100

9 571 684

100

9 573 660

100

3′adapter_nullc

1308

0.01

1405

0.01

1626

0.02

1307

0.01

Insert_nulld

2740

0.03

2833

0.03

1254

0.01

1075

0.01

5′adapter_contaminantse

108 280

1.13

71 737

0.75

35 436

0.37

26 874

0.28

Smaller_than_18ntf

20 206

0.21

38 263

0.40

21 318

0.22

10 545

0.11

PolyAg

52

0.00

67

0.00

52

0.00

82

0.00

Clean_readsh

9 440 912

98.62

9 455 761

98.81

9 511 998

99.38

9 533 777

99.58

  1. aTotal reads: total sequenced reads, which are generally required to be > 5 million.
  2. bHigh_quality: number of high-quality reads.
  3. c3′adapter_null: number of reads with no 3′ adaptor.
  4. dInsert_null: number of reads with no insertion.
  5. e5′adapter_contaminants: number of 5′ contaminants.
  6. fSmaller_than_18 nt: number of reads less than 18 nt.
  7. gPolyA: number of reads with polyA.
  8. hClean_reads: number of clean reads after removing the adaptors and contaminants. Clean reads were used in all analyses in this study.