Table 1 Primers used for real-time PCR analysis
Gene | Primers sequences | Size in base pairs |
|---|---|---|
ZO-1 | F: 5′-TGAGTTTGATAGTGGCGTTG-3′ | 298 |
R: 5′-TGGGAGGATGCTGTTGTC-3′ | ||
Occludin | F: 5′-CTAGTCGGGTTCGTTTCC-3′ | 167 |
R: 5′-GACTGATTGCCTAGAGTGT-3′ | ||
β-actin | F: 5′-TGCGGGACATCAAGGAGAAGC-3′ | 273 |
R: 5′-ACAGCACCGTGTTGGCGTAGAG-3′ |