Vector | Target | Primer | Primers for plasmid construction (5′–3′) |
---|---|---|---|
pUSG-DE | 003 | 003LF-forward-1 003LF-reverse-1 003RF-forward-1 003RF-reverse-1 | 5′ CCAATAGGATGCGGCTCT 3′ 5′ TGTACAAGTAAAGCGGCCGCAGCTTTTCACTTTTTGATGT 3′ 5′ AAATTCTACTATATCTCGACTATGATTTATTTTTGGAATA 3′ 5′ TCCTATAAAGCGACAACC 3′ |
010 | 010LF-forward-1 010LF-reverse-1 010RF-forward-1 010RF-reverse-1 | 5′ ATCTCACATACTCTGCCA 3′ 5′ TGTACAAGTAAAGCGGCCGCTTTTTTTATCAAAACTGAAG 3′ 5′ AAATTCTACTATATCTCGACTATTGTTATTTATTTGAATA 3′ 5′ ATATCCTATAATGGAAGAAG 3′ | |
TK | TKLF-forward-1 TKLF-reverse-1 TKRF-forward-1 TKRF-reverse-1 | 5′ TTGCTTTAGCTGGTAAGT 3′ 5′ TGTACAAGTAAAGCGGCCGCAAAGTGTTTATTTATTTTTC 3′ 5′ AAATTCTACTATATCTCGACAATATTGAAAATATAATTAA 3′ F2 5′ ATAGTCATGTTTGCGACCAT 3′ | |
pUS-DE | 003 | 003LF-forward-2 003LF-reverse-2 003RF-forward-2 003RF-reverse-2 | 5′ CCAATAGGATGCGGCTCT 3′ 5′ AGCTTTTCACTTTTTGATGT 3′ 5′ ACATCAAAAAGTGAAAAGCTTATGATTTATTTTTGGAATA 3′ 5′ TCCTATAAAGCGACAACC 3′ |
010 | 010LF-forward-2 010LF-reverse-2 010RF-forward-2 010RF-reverse-2 | 5′ ATCTCACATACTCTGCCA 3′ 5′ TTTTTTTATCAAAACTGAAG 3′ 5′ CTTCAGTTTTGATAAAAAAATATTGTTATTTATTTGAATA 3′ 5′ ATATCCTATAATGGAAGAAG 3′ | |
TK | TKLF-forward-2 TKLF-reverse-2 TKRF-forward-2 TKRF-reverse-2 | 5′ TTGCTTTAGCTGGTAAGT 3′ 5′ AAAGTGTTTATTTATTTTTC 3′ 5′ GAAAAATAAATAAACACTTTAATATTGAAAATATAATTAA 3′ 5′ ATAGTCATGTTTGCGACCAT 3′ |