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Table 1 SPV deletion vector pUSG-DE and pUS-DE to construct primers

From: Swinepox virus vector-based vaccines: attenuation and biosafety assessments following subcutaneous prick inoculation

Vector

Target

Primer

Primers for plasmid construction (5′–3′)

pUSG-DE

003

003LF-forward-1

003LF-reverse-1

003RF-forward-1

003RF-reverse-1

5′ CCAATAGGATGCGGCTCT 3′

5′ TGTACAAGTAAAGCGGCCGCAGCTTTTCACTTTTTGATGT 3′

5′ AAATTCTACTATATCTCGACTATGATTTATTTTTGGAATA 3′

5′ TCCTATAAAGCGACAACC 3′

010

010LF-forward-1

010LF-reverse-1

010RF-forward-1

010RF-reverse-1

5′ ATCTCACATACTCTGCCA 3′

5′ TGTACAAGTAAAGCGGCCGCTTTTTTTATCAAAACTGAAG 3′

5′ AAATTCTACTATATCTCGACTATTGTTATTTATTTGAATA 3′

5′ ATATCCTATAATGGAAGAAG 3′

TK

TKLF-forward-1

TKLF-reverse-1

TKRF-forward-1

TKRF-reverse-1

5′ TTGCTTTAGCTGGTAAGT 3′

5′ TGTACAAGTAAAGCGGCCGCAAAGTGTTTATTTATTTTTC 3′

5′ AAATTCTACTATATCTCGACAATATTGAAAATATAATTAA 3′

F2 5′ ATAGTCATGTTTGCGACCAT 3′

pUS-DE

003

003LF-forward-2

003LF-reverse-2

003RF-forward-2

003RF-reverse-2

5′ CCAATAGGATGCGGCTCT 3′

5′ AGCTTTTCACTTTTTGATGT 3′

5′ ACATCAAAAAGTGAAAAGCTTATGATTTATTTTTGGAATA 3′

5′ TCCTATAAAGCGACAACC 3′

010

010LF-forward-2

010LF-reverse-2

010RF-forward-2

010RF-reverse-2

5′ ATCTCACATACTCTGCCA 3′

5′ TTTTTTTATCAAAACTGAAG 3′

5′ CTTCAGTTTTGATAAAAAAATATTGTTATTTATTTGAATA 3′

5′ ATATCCTATAATGGAAGAAG 3′

TK

TKLF-forward-2

TKLF-reverse-2

TKRF-forward-2

TKRF-reverse-2

5′ TTGCTTTAGCTGGTAAGT 3′

5′ AAAGTGTTTATTTATTTTTC 3′

5′ GAAAAATAAATAAACACTTTAATATTGAAAATATAATTAA 3′

5′ ATAGTCATGTTTGCGACCAT 3′