Figure 2

The virus replication characteristics in cells and mice. A The swine pox virus deletion strain was observed under a fluorescence microscope at 72 h post-infection (I) Δ003-G, (II) ΔTK-G, (III) Δ010-G, and (IV) PK-15 cells. Magnification 100×. B Growth kinetics curves of Δ003, Δ010, ΔTK and wtSPV in PK-15 cell cultures. C Measurement of viral loads in PK-15 cells by qPCR. D Changes in body weight of BALB/c mice after inoculation with Δ003, Δ010, ΔTK and wtSPV. The percent body weight change was calculated from the body weights of 10 mice recorded daily after infection relative to that at day 0 (pre-infection). *P < 0.05.