Figure 5From: Oral immunization with a novel attenuated Salmonella Typhimurium encoding influenza HA, M2e and NA antigens protects chickens against H7N9 infectionProtective efficacies of the Salmonella-based influenza vaccines against LPAI H7N9 challenge. Chickens (N = 12) were immunized with Sal-vector, Sal-HA, Sal-M2e, Sal-M2e-NA or Sal-HA-M2e-NA vaccine, and 28 days later all the immunized chickens were challenged with 1.4 × 104 TCID50 H7N9 virus. The protective efficacy was determined by estimation of H7N9 viral RNA copy numbers in the cloacal swab samples of the immunized chickens (n = 10) after challenge with the lethal H7N9 virus. dpc: days post-challenge.Back to article page